This study examines the impacts of dry-weather sewer overflows (SOs) on stream water quality for three storm and three combined sewers in the Black Creek watershed, Toronto, Canada. Water samples were collected from outfalls, and from the stream at locations upstream and downstream of the outfalls. The application of fluorescent dissolved organic matter (fDOM) as a wastewater indicator was evaluated through paired bacterial monitoring with Escherichia coli (E. coli). An excitation-emission-matrix parallel factor analysis model identified the presence of wastewater derived component: C4 (tryptophan-like). Results from Kruskal-Wallis tests indicate higher concentrations of major ions (nitrate, fluoride, chloride, sulfate), dissolved organic and inorganic carbon, and C4 at the outfalls relative to the stream, however, there was no significant difference in upstream and downstream water quality. Notably, there was no significant difference in E. coli and C4 between combined and storm SOs. These results suggest, at this spatial scale, SOs have no discernible impact on stream water quality. A positive spearman’s rho between C4 and E. coli was expected; however, this was not observed, suggesting E. coli’s high variability may make it an ambiguous indicator of wastewater from dry-weather SOs. These findings suggest storm SOs in the Black Creek exhibit similar water quality impacts as combined SOs, although their downstream impacts were not observed at this spatial scale. Future work should examine the water quality impacts of dry-weather SOs at larger spatial scales including the cumulative effects of more SOs, alongside the performance of fDOM-based wastewater indicators and E. coli.