Buried valleys are an important aquifer type within glaciated landscapes. Due to complex erosion and fill history, understanding a valley’s geometry and sediment infill architecture from boreholes alone can be challenging. This study assesses the performance of shallow seismic reflection and HVSR (horizontal-to-vertical spectral ratio) methods to resolve complex bedrock valley geometry and delineation of aquifer-aquitard units in a regional flow context by comparison with a conceptual model of the valley developed from airborne frequency-domain electromagnetic (FDEM), ground-based gravity, electrical resistivity, and seismic refraction data, together with high-resolution logging of continuously cored boreholes spanning the valley fill and underlying Silurian dolostone bedrock. High-resolution S- and P-wave seismic reflection surveys were collected along two transects centered over a bedrock valley in Elora, Ontario. Reflection data were complemented by microtremor HVSR stations along each reflection profile. Seismic reflection provided high-resolution images of the bedrock and infilling sediments; however, the method could not fully resolve the sub-vertical sidewalls of the bedrock valley profile, or intra-facies sedimentary boundaries of the infill previously conceptualized using airborne electromagnetic and electrical resistivity methods. Although HVSR resonance is locally attributed to the shallow dense till unit, strong resonance was obtained from the deeper valley floor demonstrating the method’s capacity for delineation of valley thalwegs. These seismic data are highly complementary to regional airborne FDEM surveys and high-resolution core logs. A well-developed multi-dimensional model of an aquifer-aquitard flow system relies not only on 2D and 3D geophysical information but also on strategically located high-resolution continuous core logs.