Extracted peatlands often require active rewetting and re-vegetating for recovering of peatland ecosystem function and reverting carbon loss. In Canada, there has been much success in restoration efforts for bog peatlands, however, there remains considerable need for research supporting fen restoration. Our objective is to improve understanding of biogeochemical processes occurring post-restoration and the significance of vegetation community and water table in supporting peat accumulation in a fen restoration context. This study is based in southeastern Manitoba in a 2016 restored fen comprised of two restored sectors differing in water table and vegetation communities, and as reference, an adjacent natural rich fen, unrestored sector and sector under active extraction. We investigate peat physical and chemical properties in relationship with i) plant functional types, ii) above and below ground plant biomass chemistry, and iii) depth from the surface. Sampling included quadrat vegetation survey of plant functional types, peat cores down to 50 cm, and above and below ground biomass for C/N analysis. Peat cores were sectioned in 5 cm intervals and analyzed for various properties such as bulk density, organic matter, C and N content, δ13C and δ15N. We will present results comparing between sectors and specific vegetation communities (e.g., shrub vs. sedge dominated). Trends in the depth profile will be further discussed in relation to new surface peat accumulation and biogeochemical processes in the residual peat post restoration. Findings support understanding of plant-soil feedbacks and evaluating fen restoration success thus informing restoration planning and monitoring.