In-situ methods are required to extract ~97% of Alberta Oil Sands Region (AOSR) bitumen reserves. Roads required for operations intersect peatlands that occupy ~50% of the AOSR, potentially influencing water table dynamics and related ecosystem processes such as vegetation growth and carbon cycling. A road-bisected peatland complex, including shrubby and wooded-conifer fen types, was studied to assess hydrologic conditions across peatland types and sides of the road. Water table depth (WTD) and dynamics were measured over two years along transects of nested wells and piezometers, and soil cores were collected to assess peat hydrophysical properties. Hydrologic conditions differed between peatland types and sides of the road. Shrubby fen reference and upgradient sites were inundated ~20% longer than downgradient sites, with downgradient positions exhibiting deeper and more rapid WTD recession. In contrast, wooded-conifer reference sites maintained deeper median WTDs than both up- and downgradient positions, and differences between sides of the road were minimal under wetter conditions but increased during the drier year. Across both peatland types, WTD variability was greater during dry conditions and consistently higher at downgradient sites than upgradient. Differences in hydrologic behaviour among peatland types were consistent with contrasting peat hydrophysical properties, including lower transmissivity and higher bulk density in the wooded-conifer fen relative to the shrubby fen. Findings demonstrate that road-adjacent hydrologic conditions differ between peatland types and intensify under drier conditions, highlighting the importance of maintaining lateral connectivity to support peatland ecosystem function in industrial landscapes.