Inland waters, serve as intense sites for biogeochemical processes, transforming organic matter into greenhouse gas (GHG) emissions. The Prairie Pothole Region— characterized by millions of pothole wetlands, numerous agricultural reservoirs, and influenced by various agricultural practices has the potential to impact regional GHG emissions. Both agricultural reservoirs and pothole wetlands are subjected to human disturbances; however, their response in GHG fluxes may vary. Pothole waterbodies release GHG to the atmosphere via multiple pathways; however, these remain poorly understood. Research is needed to understand how GHG exchange changes with receding ponded areas and increasing exposed sediment areas. The role of nitrogen fertilizer addition for GHG release from lentic prairie systems is also poorly documented. Agricultural reservoirs may also experience increases in their GHG emissions due to elevated nutrient-trapping of DOC and fertilizer-rich runoff from the surrounding landscape. The impact of these disturbances on GHG emissions and their contribution to GHG budgets remains poorly constrained. Our research aims to address this gap by quantifying GHG fluxes from pothole wetland sediments, considering the role of sediment exposure under controlled laboratory conditions. We will investigate potential drivers including livestock-physical disturbance, and fertilizer additions. The behaviour of wetland ponds will be contrasted with agricultural reservoirs, quantifying multiple GHG flux pathways, and assessing their potential drivers using physicochemical data. This research is important to reducing regional uncertainty around GHG emissions from the Prairie landscape and informing how changing agricultural practices and future climate have the potential to alter GHG release, and C storage, for inland waters.