Hydrological observatories in northern environments are scarce. Limited resources and logistical challenges have led to a consolidation and gradual decline of circumpolar observations over the past several decades. This decline is occurring during a period of rapid environmental change, largely driven by global warming, which coincides with increased resource extraction and population growth in permafrost regions. To address concerns related to northern infrastructure and water security, the Tombstone Waters Observatory was established in 2019 in partnership with the Yukon Government and the Tr’ondëk Hwëch’in First Nation. Seven headwater streams were selected between Km 44 and Km 185 along the Dempster highway that cover three distinct ecozones within the Tombstone Mountains, Blackstone Uplands and the Ogilvie Mountains. Since 2019 we have collected a comprehensive set of hydrological, water chemistry, water stable isotope and carbon flux data within these catchments. Specific projects currently examine the influence of permafrost on surface-groundwater interactions, catchment storage and mixing, mobilization of solutes, trace metals, stream thermal regimes, surface-atmosphere exchange and carbon balances. This presentation will introduce TWO facilities and highlight select new results emerging from this work that show: 1) considerable variability in stream chemistry with serious water quality implications, 2) isotope-informed water balances indicate rapid transit of water and the importance of ground thaw as a water source, 3) stream thermal regimes are influenced by permafrost extent, and 4) there is a strong variability in fluvial carbon exports. Community partnerships and future directions will be discussed.