Mayo Lake is located within the Traditional Territory of the First Nation of Nacho Nyäk Dun (FNNND) in central Yukon. This lake provides vital habitat for wildlife and fish and supports traditional livelihoods within the community. Placer gold mining activity has expanded significantly since 2015 on some of Mayo Lake’s tributaries. Placer mines discharge sediment laden water into downstream waterbodies, reducing water quality and habitat for fish. Further, climate change has intensified rainstorms, increased forest fire frequency, and enhanced permafrost thaw in the region, all of which exacerbate sediment loading through loss of vegetation, slope failure, and runoff. Carleton University researchers and FNNND have been monitoring turbidity, suspended sediment concentration, flow, and other water quality parameters on six tributaries of Mayo Lake that are impacted by operating placer mining, historical placer mining or forest fire. These continuous monitoring data are used to characterize the streamflow and suspended sediment regimes, with a particular focus on seasonal and event-scale runoff dynamics. This research aims to improve our understanding of the potential cumulative effects of ongoing disturbances to water quality to support FNNND’s Regional Land Use Planning (LUP) efforts within their Traditional Territory. Regional LUP is a modern treaty obligation, but industrial development of the Mayo Lake watershed is accelerating without such a plan. Results of this study will contribute directly to the FNNND’s strategy for water monitoring and management within the LUP process.