The Canadian Prairie has been characterized as the most intensively managed landscape in the country. Intensive landscape modification and agricultural use, coupled with growing population, poor surface water quality, and reliance on groundwater and exotic rivers contribute to widespread water security challenges in the region. In this presentation we will discuss elements of our 7-year research project on aquatic ecosystem health in the context of social learning, linking our investigations of hydrology and biogeochemistry to knowledge mobilization in the triple loop cycle. First, we will highlight major water management challenges in the region, and what key knowledge gaps were identified through collaboration at the project outset. Second, we will outline novel strategies we used to explore these gaps, provide an overview of how we quantified impacts to aquatic ecosystem health associated with land-use and climate changes, and explain how this information was shared. Thirdly, we will reflect on the critically important third learning loop, considering the interactions between project partners and our team of researchers and exploring how science generated through the project is being used to address the question “how do we decide what is right?”. Our presentation will provide perspective to those unfamiliar with transdisciplinary research and highlight emerging biogeosciences knowledge on the Prairie landscape.