In the 17th and 18th centuries, Acadians in Atlantic Canada drained and dyked salt marshes along the Bay of Fundy, transforming them into agricultural fields. As of 2015, 77% of all salt marshes in the Bay of Fundy have been significantly modified for agricultural purposes. Maintaining this land transformation requires extensive networks of ditches to facilitate drainage and prevent the fields from reflooding. These drainage ditches occur approximately every 15 m in and around the agricultural fields and vary in size, maintenance, and other physical characteristics. Agricultural drainage ditches are greatly affected by agricultural runoff, making them hotspots for greenhouse gases (GHG), including methane (CH4), carbon dioxide (CO2), and nitrous oxide (N2O), as confirmed by numerous studies. This pilot study investigates the influences that the surrounding environmental conditions have on the GHG concentrations of agricultural drainage ditches in Sackville and Shepody, New Brunswick. The type of crop grown adjacent to the ditches (hay or corn), water temperature, air temperature, dissolved organic carbon (DOC) concentrations, and cross-sectional area of the ditches were characterized as potential influential variables in the ditch GHG concentrations. CH4 concentrations were positively influenced by DOC concentrations and cross-sectional ditch area, whereas N2O concentrations were negatively correlated to both water and air temperature. Concentrations for all three GHGs differed significantly between the six ditches sampled, likely due to the fertilizer usage from nearby fields. No consistent significant relationships were found between GHG concentrations and the type of crop grown adjacent to the ditches.