Soil moisture (SM), a critical variable for land-atmosphere interactions, is routinely available from several satellite platforms but the available spatial-temporal resolutions can be limiting factors for adoption. Opportunities to observe SM at relatively high spatial resolution have become available through the development of sensors sensitive to SM variability mounted on uninhabited aerial vehicle (UAV) platforms. This research evaluates a new L-band radiometer sensor developed by Skaha Remote Sensing Ltd., mounted to a UAV platform for routine observation of SM variation. The experiment was conducted over a single growing season at the University of Guelph’s Elora Research Station (ERS). At the ERS, weekly flights were conducted from 30-05-2023 to 24-10-2023 over a long-term tillage and crop rotation experiment, established in 1982 to investigate the impacts of both crop rotation and tillage practices (conventional vs. no tillage) on crop yield and soil health. Weekly flights of the UAV were performed carrying the L-band instrument over a sequence of plots. The L-band brightness temperature obtained from the instrument was compared to in-situ SM observations obtained from 28 different experimental plots. These plots had variable agricultural crops (soybean, corn, winter wheat, barley, alfalfa) and differing tillage management practices. The results demonstrate high and statistically significant differences in observed L-band brightness temperatures measured over the differing plots. The L-band brightness temperature variation also shows statistically significant relationships to observed in-situ SM, suggesting that the instrument will be useful for SM monitoring at relatively high spatial resolution.