The challenges associated with modern agricultural practices have led to an increased interest in sustainable agriculture. Organic farming and permaculture are two possible agricultural management practices that could lead to increased sustainability. This study aimed to fill a gap in existing literature by providing a soil quality comparison between organic farming and permaculture practices. Using plots at a community farm in Ottawa, Ontario, this study used twelve soil physical (soil temperature and soil moisture), chemical (soil pH and nutrients supply), and biological indictors (CO2 flux, decomposition rate, and FDA Test) to compare the impact of these two farming practices on soil quality. Three plots with a total of twelve sampling sites were used for each farming practice. Out of the twelve indicators, only three showed a significant difference between the two management practices. In permaculture plots soil temperature was significantly lower, soil moisture was significantly higher, and iron supply was significantly higher as compared to organic farming plots. Practices such as mulching and the use of chicken manure amendments could account for some of these differences. The findings indicate that both organic farming and permaculture have similar effect on soil properties and enhance our understanding of the impact permaculture practices on soil quality.