Environmental changes and human activities in the Sahtú watershed –including processes of colonization, mining, and sport fishing—, have led to disturbances to Sahtúgot’ı̨nę relationships with fish and Dene ts’ılı̨ (culture and way of life). In the context of the Délı̨nę Self-Government Agreement (2015), we reflect on ongoing collaborative research mapping cumulative impacts and Dene knowledge of fisheries in the Délı̨nę community, Northwest Territories. As we look at fisheries as a social-ecological system (SES), we propose adding a decolonial lens to the study of Indigenous fisheries, while gaining a better understanding of Sahtú Dene fishery relationships. In this sense, we follow a framework based on the coevolution of knowledge, drawing on Dene principles and knowledge, as well as Western knowledge. We reflect on the implications and challenges for transdisciplinary research with an emphasis on geography.